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KMID : 0861020220370050027
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Korea Journal of Herbology
2022 Volume.37 No. 5 p.27 ~ p.35
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3-Acetyl-11-Keto-Beta-Boswellic Acid from Boswellia serrata Attenuates Monosodium Iodoacetate-induced Osteoarthritis by Chondroprotective and Anti-inflammatory Effects
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Kim Min-Ju
Shin Mi-Rae
Park Hae-Jin
Roh Seong-Soo
Choi Hak-Joo
Choi Hwang-Yong
Kim Hwa-Young
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Abstract
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Objectives：3-Acetyl-11-keto-β-boswellic acid (AKBA) is a major active compound in Boswellia serrata. We
investigated the arthritic changes following AKBA administration in monosodium iodoacetate (MIA)-induced
osteoarthritis rats.
Methods：All rats were randomly divided into five groups: Normal, Control, INDO (indomethacin 2 mg/kg treated),
AKBA30 (AKBA 30 mg/kg treated), and AKBA60 (AKBA 60 mg/kg treated); drugs were given 2 weeks before MIA
injection. For all groups except the normal group, 50 μL of sterile saline with MIA (80 mg/mL) was injected into
the right knee joint 2 weeks after drug administration. The drug administration was continued for 4 weeks from 1
week after osteoarthritis induction. The histomorphological changes of knee joint cartilage were observed by H&E
staining. Also, the levels of glycosaminoglycan (GAG), cartilage oligomeric matrix protein (COMP), 5-lipoxygenase
(5-LOX), 5-LOX-activating protein (FLAP), and leukotriene B4 (LTB4) in the knee joint were determined by the
ELISA kits. The expressions of mitogen-activated protein kinases (MAPKs), inflammatory cytokines, and matrix
metalloproteinases (MMPs) in knee joint were detected by Western blot.
Results：Data show that levels of 5-LOX, FLAP, LTB4, and COMP were downregulated significantly in the AKBA
treated groups when compared to those in the Control group. On the other hand, GAG levels were significantly
elevated. As a result of Western blot, the AKBA-treated groups significantly inhibited phosphorylation of MAPKs.
In addition, significant downregulation of the expression of inflammatory cytokines and MMPs was found in the
AKBA-treated groups.
Conclusion：Our findings suggest that administration of AKBA could exert better chondroprotective and antiinflammatory effects for MIA-induced osteoarthritis rats.
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KEYWORD
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Boswellia serrata, 3-Acetyl-11-keto-β-boswellic acid, Monosodium iodoacetate, Osteoarthritis
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